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cd14 + cell-enriched fractions  (Miltenyi Biotec)


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    Miltenyi Biotec cd14 + cell-enriched fractions
    DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) <t>CD14+</t> DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.
    Cd14 + Cell Enriched Fractions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+++cell-enriched+fractions/cd14/pmc00416524-173-8-16
    Average 90 stars, based on 1 article reviews
    cd14 + cell-enriched fractions - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Lack of Phenotypic and Functional Impairment in Dendritic Cells from Chimpanzees Chronically Infected with Hepatitis C Virus"

    Article Title: Lack of Phenotypic and Functional Impairment in Dendritic Cells from Chimpanzees Chronically Infected with Hepatitis C Virus

    Journal:

    doi: 10.1128/JVI.78.12.6151-6161.2004

    DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) CD14+ DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.
    Figure Legend Snippet: DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) CD14+ DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.

    Techniques Used: Infection, Cell Culture, Incubation

    Related Articles

    Magnetic Cell Separation:

    Article Title: Human chimeric antigen receptor macrophages for cancer immunotherapy
    Article Snippet: Apheresis-derived leukopacs were subjected to elutriation using an Elutra Cell Separation System (Terumo BCT) to reduce erythrocytes, platelets, lymphocytes and granulocytes. .. Monocyte-enriched fractions were pooled and subjected to MACS CD14-positive selection (Miltenyi Biotec) according to the manufacturer’s instructions. ..

    Selection:

    Article Title: Human chimeric antigen receptor macrophages for cancer immunotherapy
    Article Snippet: Apheresis-derived leukopacs were subjected to elutriation using an Elutra Cell Separation System (Terumo BCT) to reduce erythrocytes, platelets, lymphocytes and granulocytes. .. Monocyte-enriched fractions were pooled and subjected to MACS CD14-positive selection (Miltenyi Biotec) according to the manufacturer’s instructions. ..



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    DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) <t>CD14+</t> DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.
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    DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) <t>CD14+</t> DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.
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    Image Search Results


    DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) CD14+ DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.

    Journal:

    Article Title: Lack of Phenotypic and Functional Impairment in Dendritic Cells from Chimpanzees Chronically Infected with Hepatitis C Virus

    doi: 10.1128/JVI.78.12.6151-6161.2004

    Figure Lengend Snippet: DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) CD14+ DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.

    Article Snippet: PBMCs from leukapheresis samples were used to obtain CD14 + cell-enriched fractions by magnetic bead separation (Miltenyi), and they were cultured for 5 days in medium supplemented with GM-CSF, IL-4, and different doses of chimpanzee plasma (5 or 10% plasma obtained from uninfected chimpanzees) or 10% FCS.

    Techniques: Infection, Cell Culture, Incubation